sara Ataei Nazari; abdollah mohammadi sang cheshmeh; Mohammad Reza Bakhtiarizadeh; ali assadi-alamouti; Ali Fouladi Nashta
Volume 22, Issue 3 , September 2020, , Pages 491-500
Abstract
This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental ...
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This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental treatments were 0 (control), 0.01, 0.1, 1 and 10 μg/ml of LPS in oocyte maturation medium. The cumulus oocytes complex (COC) were cultured with various concentrations of lipopolysaccharide. After 24 h of oocyte maturation, the medium was collected and the rates of oocyte maturation, cleaved oocyte and oocytes reached to blastocyst stage were analyzed. Oocyte maturation rate was lowest in the treatment with 10 μg/ml of LPS (P<0.05). Among the measured metabolites, only glucose concentration was linearlydecreased in response to increasing levels of LPS in the maturation medium (P<0.05) as treatment with 10 μg/ml of LPS had lower glucose concentration comparing to other treatments. The percentage of oocyte cleavage was significantly lower in treatment with highest level of LPS compared to other treatments (P<0.05). In addition, the treatment with 1 and 10 μg/ml of LPS significantly reduced blastocyst rate compared to control group (P<0.05). According to results of this study, lipopolysaccharide could have detrimental effects on oocyte development and these influences seems to be mediated through pathways related to energy metabolism. Acquiring managerial approaches to control LPS enhancing agents during reproductive season could prevent animal's reproductive failure.
vahidVahid mohammadi; seyed davood sharifi; Mohsen Sharafi; abdollah mohammadi sang cheshmeh
Volume 22, Issue 2 , June 2020, , Pages 271-279
Abstract
The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications ...
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The effect of feeding L-carnitine during pre-puberty on the quality parameters of fresh and frozen-thawed semen by using 12 Ross broiler breeder males (12 weeks) for 18 weeks, in a completely randomized design with three treatments (0, 250 and 500 mg / kg of L-carnitine in the diet) and four replications was performed. From the age of 26 to 29 weeks, semen collection was performed using abdominal massage. The sperms taken each time after dilution (with Beltsville diluent) were divided into two parts, one section was frozen and the other part was immediately examined. Motility (total and forward), viability, morphology, membrane functionality and lipid peroxidation parameters were evaluated. In fresh sperm, the correlation between L-carnitine and abnormalities was negative linear, and with viability was positive linear (P<0.05). Quadratic analysis was significant in forward Motility and MDA concentration (P<0.05). Birds that use diets containing L-carnitine, In terms of forward motility, viability, morphology and MDA concentrations in fresh sperm, And these traits, with the total motility and integrity of the plasma membrane of frozen sperm, were higher in comparison to the control group (P<0.05). Also, in the sperm after frozen-thawed, the correlation between L-carnitine and Motility (total and forward), viability and membrane integrity were positive linear (P<0.05), and the correlation between L-carnitine and MDA concentration was negative linear (P<0.05). The correlation between L-carnitine and Motility (total and forward), membrane integrity and MDA concentration were quadratic (P<0.05). According to the results, Dietary L-carnitine supplementation in pre-puberty improves the qualitative traits of sperm before and after freezing in the breeder broilers.
Behnam Abbaspour; seyed davood sharifi; shokoufe ghazanfari; abdollah mohammadi sang cheshmeh; Shirin Honarbakhsh
Volume 21, Issue 2 , July 2019, , Pages 279-290
Abstract
This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment ...
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This study aimed to evaluate the effect of L-arginine on qualitative characteristics and fatty acid profile of Ross 308 aged broiler breeder roosters’ sperm. Twelve breeder roosters at age of 52 week were used in a completely randomized design with three treatments and four replicates in each treatment for eight consecutive weeks. Experimental groups were consisting diets with levels of 0.52 (Ross recommendation 308), 0.68 and 0.83 present of arginine amino acids. Semen collection was performed every 14 days and in weeks 54, 56, 58 and 60 were tested. At the end of the trial, fatty acid profile of sperm was also evaluated. On week 56, semen volume in roosters with 0.68 present arginine was higher than other treatments (P<0.05). On week 60, levels of 0.52 and 0.68 percent arginine in semen volume, percentage of total and progressive motility were higher than the level of 0.83 percent of arginine (P<0.05). On week 58, the percentage of abnormal sperm were lower in 0.68 and 0.83 percent arginine treatment compared to 0.52 percent arginine (P<0.05). Semen concentration, sperm plasma membrane functionality and sperm fatty acid profile were not affected by treatments used in this study. It can be concluded that 0.68 percent of arginine (30% higher than recommendation) of diet improve some qualitative sperm parameters in aged broiler breeder roosters.
Parisa Yazdanshenas; Raana Jahanbin; Abdoullah Mohammadi Sangcheshmeh; Mehdi Aminafshar; Hossein Vaseghi Dodaran; Hamid Varnaseri; Mohammad Chamani; Mohammad Hassan Nazaran
Volume 18, Issue 1 , April 2016, , Pages 173-181
Abstract
This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing ...
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This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing zero (Control), 10-6, 10-5, 10-4, 10-3 and 10-2 M of zinc nano-complex. The samples were then freezed-thawed after 72 hours. Motility of sperm was analyzed using computer assisted sperm analysis. The highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA were not different among the groups. However, the groups containing 10-6 and 10-2 M zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.
Raana Jahanbin; Parisa Yazdanshenas; Mehdi Amin Afshar; Abdollah Mohammadi Sangcheshmeh; Hamid Varnaseri; Mohammad Chamani; Mohammad Hasan Nazaran; Mohammad Reza Bakhtiyarizadeh
Volume 17, Issue 2 , October 2015, , Pages 371-380
Abstract
The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, ...
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The effect of zinc Nano-particles (Zn- nano- complex) on bull sperm quality after freeze-thawing process studied. Ejaculates collected from four Holstein bulls twice a week. On the day of semen collection, four ejaculates were pooled and diluted with Bioxcell extender containing 0, 10-6, 10-5, 10-4, 10-3, 10-2 molar of zinc Nano-complex and frozen. After thawing, sperm motility and motion parameters, plasma membrane integrity, abnormal morphology, plasma membrane functionality and mitochondrial activity were determined. The proportion of the total and progressive motile sperm, the plasma membrane integrity and proportion of the spermatozoa with abnormal morphology was not different among groups. Zinc Nano-complex groups represented a higher plasma membrane functionality than that of control group. Moreover, our flowcytometric data suggested that spermatozoa in the groups of zinc Nano-complex possessed higher mitochondrial activity as compared with the control group. Mitochondrial activity in 10-2 was higher than the 10-6 group. In conclusion, supplementation of zn Nano-complex, can improve the plasma membrane functionality and mitochondrial activity of bull spermatozoa in a dose dependent manner without any deleterious effect on motility parameters.
Atefeh Berenjian; Seyed Davood Sharifi; Abdollah Mohammadi Sangcheshmeh; Shokoofeh Ghazanfari
Volume 17, Issue 1 , April 2015, , Pages 19-28
Abstract
Effects of chromium nano particles (NanoCr) on performance and meat quality of Japanese quail under physiological stress was determined by using 360 birds in a completely randomized design with six treatments, four replicates and 15 birds per each, during 17- 35 d of age. The tratments were ...
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Effects of chromium nano particles (NanoCr) on performance and meat quality of Japanese quail under physiological stress was determined by using 360 birds in a completely randomized design with six treatments, four replicates and 15 birds per each, during 17- 35 d of age. The tratments were negative control (non-stressed), and five groups under physiological stress which fed on diets supplemented with different levels of NanoCr (0, 200, 400, 800 and 1200 µgkg-1). Dexamethasone (0.6 mgkg-1 BW) was added to diet for induction of physiological stress. Physiological stress reduced feed intake and growth, whereas increased feed conversion ratio (FCR) and thigh and breast muscles malondialdehyde (MDA) concentration (P<0.05). Stressed birds were fed on diets containing increased levels of NanoCr linearly improved weight gain (P< 0.02), and decreaced FCR (P< 0.05), MDA levels in breast (P< 0.04), and thigh muscles (P< 0.006). The results showed that supplementation of diet with NanoCr can alleviate the negative effects of physiological stress on performance and meat quality of quails.